VOLUME ESTIMATION FROM SINGLE IMAGES: AN APPLICATION TO PANCREATIC ISLETS

Quantitative proteomic analysis of single pancreatic islets.

Technological developments make mass spectrometry (MS)-based proteomics a central pillar of biochemical research. MS has been very successful in cell culture systems, where sample amounts are not limiting. To extend its capabilities to extremely small, physiologically distinct cell types isolated from tissue, we developed a high sensitivity chromatographic system that measures nanogram protein ...

Progesterone and Cilostazol Protect mice pancreatic islets from oxidative stress induced by hydrogen peroxide

Abstract Reactive oxygen species and oxidative stress impair β-cell function and reduce insulin secretion. It has been shown that progesterone and cilostazol possess antioxidant properties. The present study was aimed to investigate in vitro pretreatment effect of progesterone and cilostazol on insulin secretion as well as their protective effects against hydrogen peroxide-induced oxidative str...

An artificial transplantation site for pancreatic islets.

Pancreatic Islets from Glucose-infused Rats

When pancreatic islets isolated from rats infused for 48-72 h with a hypertonic solution of D-glucose were incubated for two successive periods of 10 min each, in the presence first of 16.7 mM and then 2.8 mM D-[U-14Cjglucose, the total output of L-lactic acid during the second incubation was as high as that recorded during the first incubation, while the specific radioactivity of L-lactic acid...

Progesterone and Cilostazol Protect mice pancreatic islets from oxidative stress induced by hydrogen peroxide

Abstract Reactive oxygen species and oxidative stress impair β-cell function and reduce insulin secretion. It has been shown that progesterone and cilostazol possess antioxidant properties. The present study was aimed to investigate in vitro pretreatment effect of progesterone and cilostazol on insulin secretion as well as their protective effects against hydrogen peroxide-induced oxidative str...